Effect of trehalose on protein structure

Nishant Kumar Jain & Ipsita Roy

Trehalose is a ubiquitous molecule that occurs in lower and higher life forms but not in mammals. Till about 40 years ago, trehalose was visualized as a storage molecule, aiding the release of glucose for carrying out cellular functions. This perception has now changed dramatically. The role of trehalose has expanded, and this molecule has now been implicated in a variety of situations. Trehalose is synthesized as a …

Protein Sci. 2019;18:24-36.
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Preferential interactions of trehalose, L-arginine.HCl and sodium chloride with therapeutically relevant IgG1 monoclonal antibodies

Chaitanya Sudrik,Theresa Cloutier,Phuong Pham,Hardeep S. Samra &Bernhardt L. Trout

Preferential interactions of weakly interacting formulation excipients govern their effect on the equilibrium and kinetics of several reactions of protein molecules in solution. Using vapor pressure osmometry, we characterized the preferential interactions of commonly used excipients trehalose, L-arginine.HCl and NaCl with three therapeutically-relevant, IgG1 monoclonal antibodies that have similar size and shape, but differ in their surface hydrophobicity and net charge. We further characterized the effect of these …

MAbs. 2017;9:1155-1168.
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Trehalose prevents aggregation of exosomes and cryodamage

Steffi Bosch, Laurence de Beaurepaire, Marie Allard, Mathilde Mosser, Claire Heichette, Denis Chrétien, Dominique Jegou & Jean-Marie Bach

Exosomes are important mediators in intercellular communication. Released by many cell types, they transport proteins, lipids, and nucleic acids to distant recipient cells and contribute to important physiopathological processes. Standard current exosome isolation methods based on differential centrifugation protocols tend to induce aggregation of particles in highly concentrated suspensions and freezing of exosomes can induce damage and inconsistent biological activity. Trehalose is a natural, non-toxic sugar widely used …

Sci. Rep. 2016;6:36162.
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Long-term storage of DNA-free RNA for use in vaccine studies

Kathryn L. Jones, Debbie Drane & Eric J. Gowans

RNA replicons represent potential vaccine delivery vehicles, but are considered too unstable for such use. This study examined the recovery, integrity and function of in vitro transcribed replicon RNA encoding hepatitis C virus (HCV) proteins. To remove residual template DNA, the RNA was digested with TURBO DNase followed by RNeasy DNase set and purified through an RNeasy column. The RNA was freeze-dried in distilled water or trehalose, stored …

BioTechniques. 2007;43:675-681.
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Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

Shu Hui Neo, Ka Yan Chung, Jia Min Quek & Heng-Phon Too

The preservation of nucleic acids from clinical samples is critical to facilitate accurate molecular diagnosis. The use of a paper matrix, Flinders Technology Associates (FTA) Elute cards, to archive DNA and viral RNA is well-documented. However, the feasibility of FTA Elute cards for archiving serum and serum exosomal microRNAs (miRNAs) remains unclear. Here, we performed a comprehensive evaluation of FTA Elute cards for miRNA storage and recovery in …

Sci. Rep. 2017;7:16686.
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Spray dried VSV‑vectored vaccine is thermally stable and immunologically active in vivo

Steven P. Toniolo, Sam Afkhami, Michael R. D’Agostino, Brian D. Lichty, Emily D. Cranston, Zhou Xing & Michael R. Thompson

Effective vaccine delivery and coverage to rural and resource-poor countries is hindered by the dependence on cold chain storage. As such, developments of cold chain-free technologies are highly sought. Although spray dried adenoviral vectors have shown long term stability at ambient temperatures and relatively low humidity, it remains to be determined whether similar excipient formulations are applicable to other viral vectors. To address this, we have spray dried …

Sci. Rep. 2020;10:13349.
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Thermal Stabilization of Viral Vaccines in Low-Cost Sugar Films

Vincent Leung, Jonathan Mapletoft, Ali Zhang, Amanda Lee, Fatemeh Vahedi, Marianne Chew, Alexandra Szewczyk, Sana Jahanshahi-Anbuhi, Jann Ang, Braeden Cowbrough, Matthew S. Miller, Ali Ashkar & Carlos D. M. Filipe

Most currently available vaccines, particularly live vaccines, require the cold chain, as vaccine efficacy can be significantly hampered if they are not stored in a temperature range of 2–8 °C at all times. This necessity places a tremendous financial and logistical burden on vaccination programs, particularly in the developing world. The development of thermally stable vaccines can greatly alleviate this problem and, in turn, increase vaccine accessibility worldwide. In …

Sci. Rep. 2019;9:7631.
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Oral trehalose supplementation improves resistance artery endothelial function in healthy middle-aged and older adults

Rachelle E. Kaplon, Sierra D. Hill, Nina Z. Bispham, Jessica R. Santos-Parker, Molly J. Nowlan, Laura L. Snyder, Michel Chonchol, Thomas J. LaRocca, Matthew B. McQueen, Douglas R. Seals

We hypothesized that supplementation with trehalose, a disaccharide that reverses arterial aging in mice, would improve vascular function in middle-aged and older (MA/O) men and women. Thirty-two healthy adults aged 50-77 years consumed 100 g/day of trehalose (n=15) or maltose (n=17, isocaloric control) for 12 weeks (randomized, double-blind). In subjects with Δbody mass<2.3kg (5 lb.), resistance artery endothelial function, assessed by forearm blood flow to brachial artery infusion …

Aging. 2016;8:1167-1183.
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Glycemic, insulinemic and incretin responses after oral trehalose ingestion in healthy subjects

Chiyo Yoshizane, Akiko Mizote, Mika Yamada, Norie Arai, Shigeyuki Arai, Kazuhiko Maruta, Hitoshi Mitsuzumi, Toshio Ariyasu, Shimpei Ushio & Shigeharu Fukuda

Background Trehalose is hydrolyzed by a specific intestinal brush-border disaccharidase (trehalase) into two glucose molecules. In animal studies, trehalose has been shown to prevent adipocyte hypertrophy and mitigate insulin resistance in mice fed a high-fat diet. Recently, we found that trehalose improved glucose tolerance in human subjects. However, the underlying metabolic responses after trehalose ingestion in humans are not well understood. Therefore, we examined the glycemic, insulinemic and …

Nutr. J. 2017;16:9.
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Exploiting macrophage autophagy-lysosomal biogenesis as a therapy for atherosclerosis

Ismail Sergin, Trent D. Evans, Xiangyu Zhang, Somashubhra Bhattacharya, Carl J. Stokes, Eric Song, Sahl Ali, Babak Dehestani, Karyn B. Holloway, Paul S. Micevych, Ali Javaheri, Jan R. Crowley, Andrea Ballabio, Joel D. Schilling, Slava Epelman, Conrad C. Weihl, Abhinav Diwan, Daping Fan, Mohamed A. Zayed & Babak Razani

Macrophages specialize in removing lipids and debris present in the atherosclerotic plaque. However, plaque progression renders macrophages unable to degrade exogenous atherogenic material and endogenous cargo including dysfunctional proteins and organelles. Here we show that a decline in the autophagy–lysosome system contributes to this as evidenced by a derangement in key autophagy markers in both mouse and human atherosclerotic plaques. By augmenting macrophage TFEB, the master transcriptional regulator …

Nat. Commun. 2017;8:15750.
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